LKK0120 Chemical Biology / Keemiline Bioloogia
In Y2018/19, the course is will be jointly run with Dr Pirjo Spuul (Cell Biology) in Spring 2019 and will feature lab assignments (6 EAP).
In Y2017/18, the course was run in Fall 2017 as LKX0101 (3 EAP). For course materials, please visit http://www.ttu.ee/study-10 and use your username/password.
General, introduction, prebiotic chemistry (PNA etc.), DoS (cell painting, principal moment of inertia, Tanimoto similarity coefficient, Lipinski rules), BoS, Fluorine (incl. permeability, lipophilicity, P-gp, Caco-2, millicell), magic methyl effect.
The classics (BTS, blebbistatin, monostrol), cytoskeleton (intro), cell cycle (FACS).
Cytoskeleton (actin, myosin, cell polarisation and migration, chemotaxis, GTPases), chemical linkers (zero-length, non-zero-length, molecular rulers, spacers).
Cytoskeleton (MTs), PTMs (natural and unnatural), unnatural aminoacids, bioorthogonal chemistry (modifications of living cells, Staudinger reaction), biologicals (nanobodies, stability), hallmarks of cancer.
MT motors (kinesins, dyneins), cancer (types, molecular basis, stages), histological staining, fluorescent staining (organic fluorophores-chemistry).
Light microscopy (brightfield, darkfield, phase, DIC, sectioning), fluorescence microscopy (FISH, antibodies, DNA stains, organic fluorophores-classes, widefield, confocal, ExM).
Fluorescent proteins (chemistry, fusion proteins, FRET, FRAP, photoactivation, bioluminescence), superresolution techniques (3D SIM, STED, STORM, PALM), fluorescent proteins (photoconversion-chemistry), other techniques (TIRF, AFM, TEM, SEM, cryoEM).
The data on the rest of the meetings are in the password-protected study section (incl. mechanotransduction and microfluidics).